Here's a close up of Spiderman's web shooter. I thought it would have some form of cartridge much like the comic book version but apparently from what can be seen here it's just this little wristwatch sized shooter. From what I can gather by watching the trailer, Peter somehow manages to develop a new sticky composite which you can see him experimenting on below.
Here you see Peter actually experimenting, developing the new composite by mixing two components. As soon as the thing in his hand makes contact with the shooter, it immediately spits out the webbing. In one scene from the trailer, you can see Peter wandering into a room filled with strands of webbing (where he eventually gets bitten) in one of OsCorp's lab. I'm guessing Peter's dad was actually trying to replicate a spider web and make it stronger for more practical uses. Peter might have stumbled upon the formula from his dad's briefcase or something. Peter being a genius himself (which they demonstrated in a scene with Peter and Dr Connors; Peter completing a complicated equation) manages to replicate the process and tadaa! Webshooters! 
From the picture above you can see the shooters actually emitting a red glow every time he uses it. The light could probably be the effect of the chemicals reacting. I don't know why they actually made the shooters light up. Looks a wee bit tacky but I think I can live with it. I can see the Spiderman action figures; light up wrist spiderman.
Spiderman's eye protectors are somewhat gold rather than the silvery / white we're used to seeing in the comics and the previous movies by Sam Raimi. Maybe the gold reflects light better and gives Spiderman a more dramatic look when filmed. Maybe. It also looks slightly smaller than the ones we saw before. No complains there.
Cicakman. People have been on the fence with the Lizard's overall look, some even going as far as saying that he looks like a scaled down version of Godzilla. I don't know why the studio didn't go for the classic Lizard look with an elongate snout but rather a Killer Croc looking lizard? Oh well...
Can someone please explain to me why the Lizard is carrying a sling bag? It's a sling bag. Trust me. Really it is! I ain't making this shit up. Don't believe me? Go back and watch the trailer and pause/play when this scene comes up. Told ya...
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